This website is edited by the Luxembourg Institute of Science and Technology (LIST) on behalf of the consortium members within the SUPERVIR project - Establishment of a long-term monitoring system for pathogenic viruses via wastewater in Luxembourg (01.12.2022 – 30.11.2025).

The table below lists the wastewater treatment plants (WWTPs) from which samples were collected. It includes the maximum capacity and population served by each WWTP. Each entry also indicates the virus being monitored and whether surveillance is still active at the site.

Some of the data presented on this website has been gathered from previous projects conducted collaboratively by the institutes listed below. Their valuable contributions have been instrumental in making this resource possible.

01.01.2022 – 31.12.2024

One of the objectives of the VIRALERT project was to provide proof of concept that wastewater surveillance could be valid for other enteric and respiratory viruses, beyond SARS-CoV-2. In this task, LIST was responsible for conducting wastewater sampling, sample pre-treatment, and nucleic acid extraction. The development of molecular biology detection methods for Influenza and RSV viruses was done in close collaboration with LIH. Regarding the data presented on the website, RSV analyses (RT-qPCR) from March 2020 to March 2024 were performed at LIH and later at LIST, while all influenza virus (RT-digital PCR), norovirus (RT-qPCR) and enterovirus (RT-qPCR) analyses were performed at LIST. The data handling and curation were performed using informatics programs jointly developed by LIH and LIST.

01.10.2021 – 30.09.2022

The objective of the CORONAVAR project was to determine if wastewater surveillance could identify major SARS-CoV-2 variants circulating in the population, using advanced molecular biology and sequencing technologies. Sequencing analyses were carried out in close collaboration with LNS and LCSB. Although variant monitoring data are not visible on the website, part of this project's funding helped generate data for the generic monitoring of SARS-CoV-2 in the country's wastewater, conducted by LIST.

01.06.2020 – 31.03.2021

The CORONASTEP+ project aimed to assess the presence of SARS-CoV-2 in domestic wastewater and evaluate its potential for tracking virus circulation in the population at the national level. The project focused on understanding how virus shedding patterns in infected individuals affect data collected from wastewater, while also assessing the infectious hazard of virus-containing wastewater. Key methodological objectives included the development of concentration procedures for enveloped viruses (LIST), the implementation of cell culture-based infectivity tests (LIH), the analysis of stool samples (LIH & LCSB) and the use of high-throughput sequencing (LNS & LCSB). While some research outputs are not displayed on the website, the project contributed to LIST's national SARS-CoV-2 wastewater surveillance program.

01.04.2020 – 30.11.2022

The CORONASTEP project was the first LIST internal project to implement SARS-CoV-2 monitoring in wastewater in the early stages of the pandemic in Luxembourg. The main objectives were to validate the wastewater concentration method for SARS-CoV-2 and to establish RT-PCR assays with the kind support of LIH and LNS.